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ACES Facilities

Frostburg State University Facilities and Capabilities


FSU Academic Programs
Frostburg State University is committed to promoting environmental sustainability and to educating students about environmental issues. As part of its ongoing efforts to respond to workforce needs and uphold its commitment to environmental education, FSU established an undergraduate degree program in ethnobotany, one of only two programs in the United States. In addition, the University offers an interdisciplinary minor in sustainability studies.


FSU Facilities
The Appalachian Center for Ethnobotanical Studies is housed within the Compton Science Center at Frostburg State University.  The building was constructed in 2003 and houses 40 state-of-the-art laboratories for the Departments of Biology, Chemistry, Physics and Engineering. Facilities within the Biology Department include a Greenhouse, Herbarium, Arboretum, Plant Taxonomy and Dendrology lab, Plant Physiology lab, Cell Biology lab, Biotechnology Laboratory, Scanning Electron Microscopy laboratory. Chemistry Department facilities are equipped a liquid chromatography mass spectrometer, fluorometer, inductively coupled plasma emission, evaporative light scattered detection, gas chromatography mass spectrometer, and nuclear magnetic resonance spectroscopy.

The University of Maryland Center for Environmental Science’s Appalachian Laboratory is located next to the FSU campus and has partnered with FSU to conduct research related to black cohosh.  The Appalachian Laboratory has state-of-the-art laboratories for plant and soil ecology, aquatic chemistry, watershed hydrology, landscape ecology, limnology, wildlife ecology, behavioral ecology, toxicology, and conservation biology.


FSU Arboretum
The Arboretum at FSU is comprised of nearly ten acres of land and provides a growing, sustainable and safe resource for the campus and regional communities to engage in education, research, observation and contemplation while maintaining the natural setting of the mountains of Appalachia. The Arboretum is the site of projects in invasive plant removal, native plant restoration, and stream buffer and riparian restoration for Sand Spring Run, and is used for teaching, research and recreation by many faculty and students.


Tai Sophia Institute Facilities and Capabilities


Tai Sophia Academic Programs
Tai Sophia Institute is committed to integrating traditional healing arts with contemporary knowledge and shaping the future of healthcare through personal and public service. In 2002, in order to meet a growing demand for well-informed practitioners, Tai Sophia Institute established the nation’s first Master of Science degree in Herbal Medicine. The Institute recently added Graduated Certificate programs in Medical Herbalism and Herbal Studies.


Tai Sophia Clinical Facilities
Clinical Services at Tai Sophia Institute include the professional Natural Care Center, faculty-supervised student clinics and a full service herbal dispensary. The combined clinics provide approximately 40,000 treatments annually. The herbal student clinic, as part of an accredited M.Sc. program, is the only one of its kind in the United States and offers a unique opportunity to see the modern day application of Appalachian and other herbal medicines.

 http://www.tai.edu/Clinical.aspx


Tai Sophia Garden Facilities
Tai Sophia Institute’s herb gardens include a small on-site garden as well as Dr. Jim Duke’s Green Farmacy Garden. The Green Farmacy Garden is 3 miles from the campus and is home to 300+ medicinal species from around the world.

http://www.tai.edu/HerbGarden.aspx


Tai Sophia Research Facilities
As an educational facility emphasizing the application of traditional herbal medicine and acupuncture, Tai Sophia has not historically been a research institution. This is changing and the herbal medicine department, led by Dr. Andrew Pengelly and James Snow, has recently completed a short-term study on the effects of Rosmarinus officinalis on cognitive function in an elderly population. Tai Sophia has retained the specialized computer-based testing equipment in anticipation of further cognitive research.


RESOURCES AND FACILITIES AT THE USM Institute for Bioscience and Biotechnology Research (IBBR)


Environment
The University System of Maryland’s Institute of Bioscience and Biotechnology Research (IBBR) (formerly the University of Maryland Biotechnology Institute’s (UMBI) Center for Advanced Research in Biotechnology (CARB)) is a recognized research center in structural biology, biophysics, computational biology and protein design, engineering and evolution.  IBBR maintains two X-ray generators and three detectors that are shared among ~5 research groups.  Access is available through a rotating schedule. In addition, the IBBR has considerable "common" space that houses equipment and instrumentation available to all investigators and their research groups. This includes several temperature and vibration controlled rooms for crystal growth, state-of- the-art NMR facilities, mass spectrometers, preparative centrifuges, analytical ultracentrifuges, fermentation apparatus, micro-calorimetry instruments, surface Plasmon resonance units, stopped-flow spectrometers, optical spectrophotometers of for absorption, time-resolved and steady-state fluorescence, infrared and polarization measurements, freeze-dryers, incubators, microscopy facilities, and glass washing/autoclave facilities. 

Additionally, there are several specialized core facilities that are available to IBBR investigators, including a state-of-the-art plant transformation facility with several small and walk-in growth chambers, and a modern, computer-controlled greenhouse with a fully equipped head house.  The 6,000 square foot greenhouse is managed by a full time specialist, and houses most of the black cohosh plants that are used in the ACES projects. 


Laboratory
The Eisenstein laboratory has 1200 square feet of lab space in room 2220 of IBBR (CARB II), a new facility opened in 2006.  This space has three fume hoods, 4 bays with eight benches and ample commons space for refrigerators, freezers, centrifuges, incubators, PCRs, spectrometers and most other equipment readily needed for molecular biology, biochemistry, protein production and chemical analysis.  .A 100 sq. ft. cold room is located adjacent to the lab space for our use. In the past year, IBBR placed a new UPLC/MS system in our lab for analyses on the metabolite profiling of black cohosh and other medicinal plants.

A significant armamentarium of equipment is located in the Principal Investigator’s laboratory that is being used for several projects.  This equipment includes, but is not limited to: Waters Acquity UPLC system with Photodiode Array and ELSD Detectors (new 2009). Waters LCT Premiere ESI-TOF Mass Spectrometer (new 2009), Gilson semiprep HPLC system, Varian UV spectrophotometer, Wyatt/Agilent light scattering instrumentation, Varian Fluorescence Spectrometer (new 2008). GE Akta Purifier 100 (new 2008), Biocad 700E, Beckman XL-I Analytical Ultracentrifuge with interference, absorption and fluorescence optics, a Perkin-Elmer fluorimeter, MJ "DNA Engine" dual block gradient thermocycler, 5 Owl agarose gel electrophoresis systems, three power supplies, Bio-Rad electroporator, Beckman benchtop swinging bucket centrifuge, three microbalances, multiple refrigerators/freezers, New Brunswick temperature controlled incubator shaker (6L capacity), crystallization station equipped with two microscopes, Flash chromatography/SPE equipment.


Major Common Equipment
There are many shared resources and common equipment at the USM’s IBBR that are available to all research groups: X-ray equipment (two R-axis, one MAR image plate), three Bruker NMR instruments (Avance 600-cryo/Avance 600/DMX500). Applied Photophysics (APL) SX18 (recently refurbished and upgraded) stopped-flow spectrometer, KinTek quench flow system, five Microcal ITC and DSC microcalorimeters, three large capacity freeze dryers, multiple rotovaps, and a Voyager MALDI-TOF MS for routine protein mass measurements.  Additionally, each floor is equipped with photo-documentation systems for crystallization and electrophoresis work, a Super-Q water system, sonication and French press cell lysis equipment, a speed vac, and a preparative ultracentrifuge.  Institutionally, there exists on site the capacity to perform fermentation from 0.5 to 50 L growth volumes with appropriate centrifuges for cell harvesting.