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Cloning the Wound Inducible Ribonuclease Promotor from Tomato

Honor Student Cloning the Wound Inducible Ribonuclease Promotor from Tomato

Department: Biology
Symposium Year: 2013
Student(s): Kaitlin Green
Faculty Mentor(s): Dr. David Puthoff

Plant wounding can have a dramatic role in whether or not a plant survives. Many of these wounds are made by attacking insects and other pests. As the world population soars, the losses of crop yields from wounding by insects and other pests must be slowed down or stopped. Continued and over-use of insecticides is not very environmentally conscious. In order to provide a set of tools for future researchers so that they can try transgenic approaches to plant protection the wound-inducible promoter of tomato’s ribonuclease gene will be cloned and inserted in front of the GUS gene in the plant transformation vector pBI121. PCR primers will be design to amplify about 1000bp of tomato DNA containing the ribonuclease gene promoter. The promoter will then be inserted using restriction sites engineered onto the ends of the promoter into the pBI121 vector.

 

 

 

Present in 2014

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