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Frostburg State University

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Gender Determination of Small Mammals using PCR and Electrophoretic Techniques

Honor Student Gender Determination of Small Mammals using PCR and Electrophoretic Techniques

Department: Biology
Symposium Year: 2012
Student(s): Mark Jackson
Faculty Mentor(s): Dr. William Seddon

Genomic DNA will be isolated from a number of small mammal species.  Animals will be assigned a gender based on examination of external and internal morphology.  DNA isolation will be performed using the Qiagen DNAeasy procedure for tissues.  PCR will be used to amplify an intron common to both the X and Y chromosomes, and PCR products will be analyzed using gel electrophoresis.

 

 

 

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