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Histological Comparison of Two Mammalian Organs

Honor Student Histological Comparison of Two Mammalian Organs

Department: Biology
Symposium Year: 2013
Student(s): Richard Eby
Faculty Mentor(s): Dr. Karen Keller

 

The goal of this procedure was to utilize two different histological staining techniques on two types of rat tissue, liver and spleen, and to observe which structures could be clearly identified with the different stains. Hematoxylin and Eosin (H&E) and the Periodic Acid/Schiff (PAS) techniques were chosen. Hepatocytes were clearly visible with both stains, but H&E stained the nuclei dark purple and cytoplasmic contents pink. PAS stained the glycogen stored in the hepatocytes, which is indicative of the crucial metabolic role of the liver. Lymphocytes present in the spleen tissue stained a dark purple with H&E because of their large nuclei and the erythrocytes stained light pink. The abundance of lymphocytes highlights the important immune role of this organ and the abundance of erythrocytes highlights the fact that the spleen is a reservoir for blood cells. PAS stained the carbohydrate-rich splenic capsule. The procedure successfully demonstrated the potential of the two staining techniques to make important cellular structures visible.

 

 

 

Present in 2014

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