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Histological Comparison of Two Regions of Mammalian Brain

Honor Student Histological Comparison of Two Regions of Mammalian Brain

Department: Biology
Symposium Year: 2013
Student(s): Joshua Elmore
Faculty Mentor(s): Dr. Karen Keller

The purpose of this experiment was to determine which staining technique, Hematoxylin and Eosin (H&E) or Periodic Acid/Schiff (PAS) was the most effective for visualization of cellular structures in mammalian brain tissue from Rattus norvegicus. Sections of the cerebrum and cerebellum were staining with both procedures.  Comparisons between the staining techniques were made by identifying visible cellular components in cerebrum and cerebellum sections.  Both regions of the brain produced similar results for the different staining methods.  In the H&E stained sections, glial cells and blood vessels were distinguishable because nuclei and basement membranes stained darkly.  In the PAS stained sections, neurons were easily distinguished because the myelin sheaths stained bright pink.  Staining with H&E appeared to produce a clearer overall image of the tissue, while the staining with the PAS method was much better for visualizing neurons within both brain regions.

 

 

 

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