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Symposium Year: 2013
Student(s): Honorine Ajuata
Faculty Mentor(s): Dr. Rebekah Taylor
Cryptopatches (CPs) are part of the murine intestinal immune compartment. The goal of this study was to locate these microscopic tissues by sectioning, staining, and examining mouse small intestine under the microscope. In this project, a fresh tissue sample from a mouse intestine was quick-frozen in freezing medium. The sample was then sliced with a cryostat into thin sections, 5 to 7 micrometers in thickness. Hematoxylin, a dye that attaches to negatively charged cell parts, and eosin, a dye that attached to positively charged cell parts, was used in staining the slides. After staining and mounting, the slides were viewed under a light microscope. Photos of immune structures in the mouse small intestine were taken for observation.
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