You're seeing this message because you're using an older version of Internet Explorer that is unsupported on our website. Please use these links to upgrade to a modern web browser that fully supports our website and protects your computer from security risks.

Mozilla Firefox

Mozilla Firefox

Google Chrome

Google Chrome

Internet Explorer 8

Hide this message

FSU Tag Line
Header Image
 
Using PCR to Amplify and Clone a Tissue Specific Promoter from Melon

Honor Student Using PCR to Amplify and Clone a Tissue Specific Promoter from Melon

Department: Biology
Symposium Year: 2013
Student(s): Jeffrey Schramm
Faculty Mentor(s): Dr. David Puthoff

 

Plants respond to injury with a battery of responses. Many times these injuries come from plant pests such as insects and predators. It is well known that humans depend on plants for food. In addition, environmental concerns dictate that we as humans need to find additional ways to protect our food source from predators like insects. These methods need to be more environmentally friendly as compared to insecticides. Promoters control when and where a gene is turned on or off. Having a toolbox of promoters will allow the mixing and matching of genes with control switches (promoters) to target gene-encoded proteins to specific areas of plants that are being attached. A phloem specific promoter will be cloned from melon. Using PCR amplification, approximately 1000 base pairs of the phloem specific promoter will be cloned into the pBI121 plant expression vector. This vector can them be used to monitor the promoter’s activity in important crop plants such as tomato.

 

 

 

Present in 2014

Click for more information on how you can present in the next Symposium!