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Compton Science Center 212
General Biology I, Plant Physiology, Cell Biology, Molecular Biology
My area of research focuses on plant interactions with their pathogens with an especial focus on the interactions with insect pests. In addition, studies on insect pest prevalence and abundance, along with population genetic analysis will help provide the best strategies for pest elimination. By conducting studies that examine plant gene expression changes I hope to not only elucidate signaling mechanisms used by plants during defense reactions but also to identify genes to be used to in protecting plants from the stresses of pathogen attacks. These genes may come from wild relatives or other species. One specific plant pest that is of interest is whiteflies. These phloem feeding insects remove photoassimilates along with vectoring viruses. The long term goal of experiments will be to breed and create plant germplasm that is resistant to or tolerant of these devastating pests.
Lennon, K.A. and Puthoff, D.P. (2013). Take-home active learning exercises may result in learning gains equivalent to in-class active learning, with both superior to traditional, low- structured lecture in Introductory Biology. J. Microbiology and Bio. Education DOI: http://dx.doi.org/10.1128/jmbe.v14i1.588
*Bowman, M. and Puthoff, D.P (2012) 3 sequences from Brassica rapa were published to GenBank Accession numbers: JX000229.1, JX000230.1, JX000231.1
Puthoff, D.P., Holzer, F., Perring, T. and Walling, L.L. (2010). Tomato Pathogenesis-related Protein Genes are Expressed in Response to Trialeurodes vaporariorum and Bemisia tabaci Biotype B Feeding. J. Chemical Ecology 36(11):1271-85.
Puthoff, D.P., Neelam, A., Ehrenfried, M.L., Scheffler, B.E., Ballard, L., Song, Q., Campbell, K.B., Cooper, B., and Tucker, M.T. (2008). Analysis of expressed sequence tags from Uromyces appendiculatus hyphae and haustoria and their comparison to sequences from other rust fungi. Mycology. 98(10):1126-1135.
Smigocki, A., Ivic-Haymes, S., Puthoff, D.P. and Zuzga, S. (2008). Recent advances in functional genomics for sugar beet (Beta vulgaris L.) improvement: progress in determining the role of BvSTI in pest resistance in roots. Sugar Tech 10(1): 91-98.
Puthoff, D.P., Ehrenfried, M.L., Vinyard, B.T. and Tucker, M.L. (2007). GeneChip profiling of transcriptional responses to soybean cyst nematode, Heterodera glycines, colonization of soybean roots J. Exp. Bot. 58(12): 3407-3418.
2013. Poster. Lennon, K.A. and Puthoff, D.P. Take-home active learning exercises may result in learning gains equivalent to in-class active learning, with both superior to traditional, low- structured lecture in Introductory Biology. American Society for Microbiology – Conference for Undergraduate Education, Denver, CO.
2012. Poster. *Bowman, M. and Puthoff, D.P. Candidate gene for Anthocyaninless (anl) of Brassica rapa. Mid-Atlantic Plant Molecular Biology Society. Laurel, MD
2011. Poster. *DeCapite, A., *Lancaster, T. and Puthoff, D. Elucidation of terpenoid medicinal compound levels in Black cohosh (Actaea racemosa) rhizomes in response to plant defense signaling molecules. Mid-Atlantic Plant Molecular Biology Society. Laurel, MD
2011. Poster. Vickers, A., Brosi, S., Howell, J. and Puthoff, D. Quantification of medicinal copoinds in vegetative and reproductive Actaea racemosa. American Society of Plant Biologists, Minneapolis, MN
2010. Poster. *Lancaster, T. and Puthoff, D.P. Methods for Increasing Levels of Terpenoid Active Ingredient(s) in Black Cohosh (Actaea racemosa) and Elucidation of Their Role in Plant Defense. Mid-Atlantic Plant Molecular Biology Society. Laurel, MD