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Dr. David P. Puthoff - Biography

Research Interests

My area of research focuses on plant interactions with their pathogens with an especial focus on the interactions with insect pests. In addition, studies on insect pest prevalence and abundance, along with population genetic analysis will help provide the best strategies for pest elimination. By conducting studies that examine plant gene expression changes I hope to not only elucidate signaling mechanisms used by plants during defense reactions but also to identify genes to be used to in protecting plants from the stresses of pathogen attacks. These genes may come from wild relatives or other species. One specific plant pest that is of interest is whiteflies. These phloem feeding insects remove photoassimilates along with vectoring viruses. The long term goal of experiments will be to breed and create plant germplasm that is resistant to or tolerant of these devastating pests.

 

Recent Publications and Abstracts
 

  • Puthoff, D.P., Neelam, A., Ehrenfried, M.L., Scheffler, B.E., Ballard, L., Song, Q., Campbell, K.B., Cooper, B. and Tucker, ML (2008). Analysis of expressed sequence tags from Uromyces appendiculatus hyphae and haustoria and their comparison to sequences from other rust fungi. Mycology. DOI: 10.1094/PHYTO-98-10-1126.
  • Smigocki, A., Ivic-Haymes, S., Puthoff, D.P. and Zuzga, S. (2008). Recent advances in functional genomics for sugar beet (Beta vulgaris L.) improvement: progress in determining the role of BvSTI in pest resistance in roots. Sugar Tech. 10(1): 91-98
  • Puthoff, D.P., Ehrenfried, M.L., Vinyard, B.T. and Tucker, M.L. (2007). GeneChip profiling of transcriptional responses to soybean cyst nematode, Heterodera glycines, colonization of soybean roots. Journal of Experimental Botany. 58(12):3407-3418.
  • Puthoff, D.P. and Smigocki, A.C. (2007). Insect feeding-induced differential expression of Beta vulgaris root genes and their regulation by defense-associated signals. Plant Cell Reports 26: 71-84.
  • Giovanini, M., Saltzmann, K., Puthoff, D.P., Gonzalo, M., Ohm, H., Williams, C.E. (2007). A novel wheat gene encoding a putative chitin-binding lectin is associated with resistance against Hessian fly. Molecular Plant Pathology 8(1): 69-82.
  • Mazarei, M., Elling, A.A., Maier, T.R. Puthoff, D.P. and Baum, T.J. (2007).GmEREBP1 is a transcription factor activating defense genes in soybean and Arabidopsis. Molecular Plant-Microbe Interactions 20(2): 107-119.
  • Giovanini, M.P., Puthoff, D.P., Nemacheck, J.A., Mittapalli, O., Ohm, H.W., Shukle, R.H. and Williams, C.E. (2006). Gene-for-Gene defense of wheat against the Hessian fly lacks an oxidative burst. Mol. Plant-Microbe Inter. 19(9): 1023-1033. Featured on print cover.
  • Subramanyam, S., Sardesai, N., Puthoff, D.P., Meyer, J., Nemacheck, J.A., Gonzalo, M. and Williams, C.E. (2006). Expression of two wheat defense-responsive genes, Hfr-1 and Wci-1, under biotic and abiotic stresses. Plant Science 170: 90-103.
  • Puthoff, D.P., Nemacheck, J.A. Sardesai, S., Subramanyam, S., and Williams, C.E. (2005). Hfr-2, a wheat cytolytic toxin-like gene, is up-regulated by virulent Hessian fly larval feeding. Molecular Plant Pathology 6(4): 411-423.
  • Puthoff, D.P. and Smigocki, A.C. (2005). Sugar beet (Beta vulgaris L.) genes regulated by sugar beet root maggot (Tetanops myopaeformis) infestation. Proceedings of the American Society of Sugarbeet Technologists.
  • Mazarei, M. Lennon, K.A., Puthoff, D.P., Rodermel, S.R., and Baum, T.J. (2004). Homologous soybean and Arabidopsis genes share responsiveness to cyst nematode infection. Molecular Plant Pathology. 5(5): 409-423.
  • Puthoff, D.P., Nettleton, D., Rodermel, S.R., and Baum, T.J. (2003). Arabidopsis gene expression changes during cyst nematode parasitism revealed by statistical analyses of microarray expression profiles. Plant J. 33: 911-921. "Highlighted by Faculty of 1000" -Genome Biology
  • Mazarei, M., Lennon, K.A., Puthoff, D.P., Rodermel, S.R. and Baum, T.J. (2003). Expression of an Arabidopsis phosphoglycerate mutase homologue is localized to apical meristems, regulated by hormones, and induced by sedentary plant-parasitic nematodes. Plant Mol. Biol. 53:513-530.
  • Mazarei, M., Puthoff, D.P., Hart, J.K., Rodermel, S.R. and Baum, T.J. (2002). Identification and characterization of a soybean Ethylene-Responsive Element Binding Protein gene whose mRNA expression changes during soybean cyst nematode infection. Mol. Plant-Microbe Inter. 15:577-586.
  • Gallie, D.R., Fortner, D., Peng, J., and Puthoff, D. (2002). ATP-dependent hexameric assembly of the heat shock protein Hsp101 involves multiple interaction domains and a functional C-proximal nucleotide-binding domain. J. Biol. Chem. 277: 39617-39626.